Peptide calculator: reconstitution and dosing

What is the volume of your syringe?

Select the peptide amount

How much bacteriostatic water are you adding?

How much peptide do you need per dose?

To reach a dose of 500 mcg, draw the syringe to 10

The blue fill shows the value to which you draw the plunger The scale is in insulin syringe units (U-100)

U-100 means 100 units = 1 ml. Tall marks are tens, short marks indicate individual units.

Proper peptide reconstitution

Our peptides are supplied in lyophilized (freeze-dried) form, which is resistant to short-term temperature fluctuations, for example during transport. At room temperature, peptides in powder form remain stable for several weeks. It is, however, important to ensure proper storage to maintain the required premium quality.

Before being used for research purposes, the lyophilized powder must be reconstituted, that is, a solution must be created by mixing the powder with a suitable solvent.

Correct reconstitution is essential for maintaining the potency and bioactivity of peptides, and therefore for obtaining accurate and reliable results in further research and experiments.

Start by preparing your workspace

As with all research work, absolute sterility and cleanliness are essential when preparing the environment for peptide reconstitution. Before starting the process, wash your hands thoroughly and put on sterile protective clinical gloves and goggles. Make sure you have all the necessary supplies ready: - Lyophilized peptide in a glass vial - Sterile syringe - Solvent (bacteriostatic water) - Gloves and safety goggles A clean, sterile environment is critical for accurate research results.

Prepare the peptide and solvent

Choosing the right solvent is a critical step in peptide reconstitution and depends on the specific properties of each peptide. At Molequa® we recommend bacteriostatic water, which is optimal for this process. Before starting reconstitution, it is essential to let both the peptide powder and the solvent, which have been stored in the freezer, warm up to room temperature. Using cold solvent could disrupt the entire process. Next, carefully remove the central section of the metal cap from the vial and thoroughly clean the top with an alcohol swab to ensure sterility. Repeat this procedure when opening the solvent bottle.

Adding the solvent

Precise handling is critical when adding the solvent to the lyophilized peptide. Using a sterile syringe, draw up the required amount of solvent and slowly inject it into the vial containing the peptide powder, tilted at a 45° angle so the liquid runs down the inner wall. Perform this step carefully to minimize the formation of bubbles and foam, ensuring uniform dissolution of the peptide.

Storing peptide solutions

Storage of lyophilized peptide powder requires a temperature of at least +4 °C, and long-term storage at −20 °C or lower. With peptide solutions, the shelf life is limited. Freezing aliquots will extend the peptide's shelf life, peptide solutions are generally stable for 3 or more weeks at +4 °C and 3 to 4 months at −20 °C. Avoid repeated freezing and thawing, however, as this can significantly degrade peptides.

Our goal is to provide researchers with expert guidance and high-quality research peptides intended exclusively for scientific use. For this reason, our instructions are purely informational; human consumption is prohibited.

Frequently asked questions

How much bacteriostatic water should I add to a peptide?

It depends on the concentration you want. For example, 5 mg of peptide with 2 ml of water gives 2.5 mg/ml. Use the calculator above for the exact volume. For research use only.

How do I convert milligrams to units (U-100)?

A U-100 insulin syringe has 100 units per 1 ml. From the solution concentration and target dose, the calculator works out how many units to draw.

Which water should I use for reconstitution?

Bacteriostatic water (0.9% benzyl alcohol) is standard; after the first puncture it keeps the solution stable for typically 28+ days at 2 to 8 °C.

How long does a reconstituted peptide last?

Lyophilized powder is stable 2 to 3 years at 2 to 8 °C. After reconstitution the literature recommends use within 28 days, refrigerated and protected from light.